Specifications PROTEEMA columns

Applicability: Natural and synthetic Proteins, Peptides, Enzymes, Gelatins/Collagens
Eluents: Water, water with salt/buffer; pH: 2 -9
Material: Special modified silica
Maximum Pressure: 150 – 200 bar (2180 – 2900 psi), depending on porosity
Maximum Temperature: 70° C
Maximum Flow Rate: (8 mm I.D.) 3 ml/min
Particle Size: 3 µm, 5 µm
Molecular Weight Range: 100 to 7 500 000 Da

Standard Columns (others on request)

Separation Range [Da]*    Single Porosity Columns
100 – 150 000                             100 Å
1 000 – 1 200 000                      300 Å
10 000 – 7 500 000                   1 000 Å
* based on protein molecular weight, for other water soluble macromolecules (e.g. pullulane) separation range ends at 50 000, 400 000, and 2 500 000 Da.

Separation Range

Calibration Curves